Device

Part:BBa_J100210:Design

Designed by: Monica Prudencio   Group: Campbell M Lab   (2015-06-02)


tCloneRed with RS4 Transcriptional Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 761
    Illegal AgeI site found at 873
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Appropriate sticky ends had to be added to the RS4 sequence in order to anneal with those created by digestion of the destination vector with BsaI.


Source

The destination vector is part J119361. The sequence of the riboswitch comes from the paper "De novo design of a synthetic riboswitch that regulates transcription termination," by Wachsmuth, et al. (referred to as "RS4" in that paper; doi:10.1093/nar/gks1330).

References